Check sequenced clones against the plasmid they were meant to be, and see exactly where they differ.
Drop in the GenBank or FASTA files your vendor sent back — one per clone, or several at once. Nothing is uploaded anywhere; the files are read in this browser and compared on your own machine.
A whole-plasmid read starts at an arbitrary base and may be on either strand. That is not a difference, so each clone is placed on the reference first — the strand and start are reported, then ignored.
Every mismatch is named by the feature it lands in, because “a substitution in the coding sequence” is a decision and “a substitution at 2,341” is a coordinate.
A clone that is not this plasmid at all is said to be unplaced, never aligned into a wall of false mismatches.
Constructs you export from the Cassette and Multigene screens are kept here as references, so a design made today can be checked against reads that arrive next week.