GG Assembler
indexing…
Each panel offers only the parts whose overhangs fit that position.
The page opens on a worked assembly picked from your library, so you can see a finished construct straight away. Change any panel to make it yours. Type 3, 4 and 8 can be split (3a+3b, 4a+4b, 8a+8b) with the buttons in the panel header. 8a+8b is the chromosomal-integration configuration. 2·3·4 and 6·7·8 collapse a whole column into one composite slot, for parts that already span those positions. Click an arc on the ring to jump to that part's panel. The strip below lists every junction overhang; one shown in red can cross-match another and misligate. Search by annotation in the box above rather than scrolling a dropdown: type tdh to see every TDH promoter and terminator in the library, with the position each one fills. Pick one and it asks before filling that slot. Export .gbk downloads the predicted plasmid. Everything shown is simulated from the real sequences — nothing is assumed from a file name.
Upstream
 
— nothing assembled yet
Junction overhangs
pick a part in every slot to see the junctions
Downstream
Reaction
Enzyme—
Cycling30× (37 °C 5′ / 16 °C 5′)
Each part20 fmol
Selection—
Start from a cassette

Pick a construct you already have. Its sequence is matched against every part in the library and the panels are filled in, so you can change one and rebuild — rather than picking all eight again.

Sweep a position

Hold the design still and vary one part. Every candidate is built and reported, including the ones that fail — a promoter with an internal site is a result, not an omission.

 
Reaction setup