Find a protein in UniProt and write it as a coding sequence that carries none of the kit’s sites.
This is the only screen that uses the network. Everything else reads your own folder of GenBank files and works offline.
The DNA is chosen codon by codon, never using a codon that would spell a BsaI, BsmBI, BbsI or NotI site. That is not the same as domesticating afterwards — a synonymous change made to remove one site can spell another two codons along.
Codon usage defaults to S. cerevisiae; pick E. coli for a part you will express there. Both are the classical preferred-codon orderings for highly expressed genes — an ordering, not a frequency table, so no CAI is claimed.
Untick an enzyme to allow its site. Useful when the part is going somewhere that never sees that enzyme, and the alternative is a run of rare codons.
The result is a gene to order, not the organism’s own sequence. Take it to the Part plasmid screen to add the BsaI ends.