
Chimera Buster
======

This package takes concensus fasta files from the MrHamer pipeline and eliminates chimeric reads by comparing the UMI sequences and finding any matches in the 5' or 3' UMIs and keeping the sequence that has the highest prevalence.

The code is Python 3.

Installation
------------
$pip install Chimera_Buster

Example
--------
$Chimera_Buster [options] input_final_name input_prelim_name output_file_prefix
positional arguments:

Arguement		Function
input_file_name		Designates clusters_concesus.fasta file from the clustering_consensus folder to be 			filtered. This is required.
input_prelim_name	Designates clusters_concesus.fasta file from the clustering folder to be filtered. 			This is required..
output_file_prefix	Designates output file prefix. This is required.

options:
Arguement		Function
-h, --help		shows this help message and exit
-m int, --mismatch int	Designates the maximum number of mismatched/indel bases allowed. Default is 1.

    
License
--------
MIT - Copyright (c) 2024 Jessica Lauren Albert

