Metadata-Version: 2.1
Name: bed2idt
Version: 1.2.0
Summary: Converts primer.bed files to IDT ordering templates
License: CC-BY-SA-4.0
Author: ChrisKent
Author-email: chrisgkent@gmail.com
Requires-Python: >=3.10,<4.0
Classifier: License :: Other/Proprietary License
Classifier: Programming Language :: Python :: 3
Classifier: Programming Language :: Python :: 3.10
Classifier: Programming Language :: Python :: 3.11
Requires-Dist: typer[all] (>=0.11.1,<0.12.0)
Requires-Dist: xlsxwriter (>=3.1.2,<4.0.0)
Description-Content-Type: text/markdown

# `bed2idt`

**Usage**:

```console
$ bed2idt [OPTIONS] COMMAND [ARGS]...
```

**Options**:

* `--version`: Shows the current bed2idt version.
* `--install-completion`: Install completion for the current shell.
* `--show-completion`: Show completion for the current shell, to copy it or customize the installation.
* `--help`: Show this message and exit.

**Commands**:

* `plates`
* `tubes`

## `bed2idt plates`

**Usage**:

```console
$ bed2idt plates [OPTIONS] BEDFILE
```

**Arguments**:

* `BEDFILE`: The path to the bed file  [required]

**Options**:

* `--output PATH`: The output location of the file. Defaults to output.xlsx  [default: output.xlsx]
* `--splitby [pool|ref|none]`: Should the primers be split across differant plate  [default: pool]
* `--fillby [rows|cols]`: How should the plate be filled  [default: cols]
* `--plateprefix TEXT`: The prefix used in naming sheets in the excel file  [default: plate]
* `--force / --no-force`: Override the output directory
* `--randomise / --no-randomise`: Randomise the order of primers within a plate
* `--help`: Show this message and exit.

## `bed2idt tubes`

**Usage**:

```console
$ bed2idt tubes [OPTIONS] BEDFILE
```

**Arguments**:

* `BEDFILE`: The path to the bed file  [required]

**Options**:

* `--output PATH`: The output location of the file. Defaults to output.xlsx  [default: output.xlsx]
* `--scale [25nm|100nm|250nm|1um|5um|10um]`: The conc of the primers  [default: 25nm]
* `--purification [STD|PAGE|HPLC|IEHPLC|RNASE|DUALHPLC|PAGEHPLC]`: The purification of the primers  [default: STD]
* `--force / --no-force`: Override the output directory
* `--help`: Show this message and exit.

